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GlcNAcylated proteins on gel

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Assistant professor of biochemistry Michael Boyce and his colleagues have developed a new method for detecting one relatively short-lived but crucial cell signaling event: the attachment of a particular sugar, called GlcNAc, onto proteins inside the cell. Two protein samples are labeled through their GlcNAc sugars with green and red fluorescent dyes, and then separated on a gel. When the "GlcNAcylated" proteins are the same in both samples, the dyes overlap and show yellow, but when they differ between samples because a signal has occurred, they show red or green only. "Now that we have this tool, we can use it to find important GlcNAcylation events in a wide variety of experiments," Boyce said. CITATION: "A novel chemical glycoproteomics platform reveals O-GlcNAcylation of mitochondrial voltage-dependent anion channel 2." Cell Reports, Oct. 3, 2013